Journal: Genetics and Molecular Research

Article Title: Characterization of yolk sac proteins of Bos indicus cattle embryos

doi: 10.4238/2012.november.14.1

Figure Lengend Snippet: Figure 2. Membranes after ECL showing the molecular weight (MW) and samples of the yolk sac of embryos 2 and 3 at an estimated gestational period from 30 to 40 days. Red arrows indicate the α-fetoprotein in the samples 2:03 with a molecular weight of 70 kDa.

Article Snippet: The secondary antibody was also diluted in TTBS and applied to the membrane for 1 h. Mouse anti-human α-fetoprotein monoclonal antibody (AFP; Serotec, MCA1863HT), mouse anti-human transferrin antibody (BD, 612124) and rabbit anti-human α-1-antitrypsin polyclonal antibody (A1AT; Abcan, AB922) were applied as primary antibodies, and anti-mouse- and anti-rabbitperoxidase conjugate as secondary antibody (GE, NIF825).

Techniques: Molecular Weight

Journal: Frontiers in Physiology

Article Title: Pathogenesis of Molar Hypomineralisation: Hypomineralised 6-Year Molars Contain Traces of Fetal Serum Albumin

doi: 10.3389/fphys.2020.00619

Figure Lengend Snippet: A molecular approach for timestamping exposures of serum albumin to dental enamel. (A) Schematic showing three hypothetical stages where albumin could become incorporated in enamel on a 6-year molar ( yellow spot ), as considered in this study. These albumin exposures are designated developmental (0–3 years), eruptive (6 years), or artefactual during subsequent extraction, as indicated. (B) Serum levels of serum albumin (ALB; dark blue ) and alpha-fetoprotein (AFP; cyan ) in healthy individuals aged from 3-months postconception through to 1-year old, as indicated. Data were collated and averaged from 9 population studies as outlined in Methods and . It can be seen that at birth ( dotted line ), ALB is about 600-fold more concentrated than AFP. (C) The adult/fetal isoform ratio for serum albumin ( black line ), as derived from (B) , rises quasi-linearly through to 6-months of age ( cream box ). Sensitivity limits for the AFP immunoblot assay reached the early-post-natal period ( cyan box ). Note the isoform ratio, which spans 6-orders of magnitude (cf. log scale), offers strong resolution of age in early infancy.

Article Snippet: Human albumin (Sigma), alpha-fetoprotein (Lee Biosolutions), recombinant alpha-fetoprotein and albumin (both tagged with glutathione S-transferase; from Abnova), and rabbit polyclonal antibodies to human albumin (PAB10220 from Abnova), alpha-fetoprotein peptide (PAB12795 from Abnova) and whole alpha-fetoprotein (H00000174-D01 from Abnova), were obtained commercially.

Techniques: Extraction, Derivative Assay, Cream, Western Blot

Journal: World Journal of Stem Cells

Article Title: Patient-derived induced pluripotent stem cells with a MERTK mutation exhibit cell junction abnormalities and aberrant cellular differentiation potential

doi: 10.4252/wjsc.v16.i5.512

Figure Lengend Snippet: Generation and identification of the human induced pluripotent stem cells derived from the retinitis pigmentosa patient. A: Timeline of human induced pluripotent stem cell generation; B: Imaging by phase-contrast microscopy. Scale bar = 200 μm; C: Karyotype analysis of the healthy control (left) and retinitis pigmentosa patient (right); D: Flow cytometry of pluripotency markers SSEA-4 and TRA-1-81; E and F: Immunostaining of pluripotency markers OCT4, NANOG, SOX2, and SSEA4 of the healthy control and retinitis pigmentosa patient. Scale bar = 20 μm; G and H: In vitro differentiation of control (G) and patient (H) induced pluripotent stem cells into three germ layers, endoderm (AFP+), mesoderm (α-SMA+) and ectoderm (GFAP+). Scale bar = 20 μm. PB: Peripheral blood; PBMC: Peripheral blood mononuclear cell; iPSC: Induced pluripotent stem cell.

Article Snippet: Following primary antibodies were used: OCT4 (Cat. #ab18976; Abcam), SOX2 (Cat. #sc-365823; Santa Cruz), NANOG (Cat. #ab80892; Abcam), SSEA4 (Cat. #ab16287; Abcam), GFAP (Cat. #HPA056030; Sigma), α-SMA (Cat. #A5228; Sigma), AFP (Cat. #MAB1368; R&D System).

Techniques: Derivative Assay, Imaging, Microscopy, Control, Flow Cytometry, Immunostaining, In Vitro